| 質粒名稱: | pBABE-neo largeTcDNA |
|---|---|
| 出品公司: | 愛迪基因 |
| 目錄編號: | 1780 |
| 存儲實驗室: | Bob Weinberg |
|
載體骨架基本信息 |
|
| 載體名稱: | pBABE-neo |
| 載體出品公司: | -- |
| 載體抗性: | 氨芐青霉素 |
| 空載體大小: | 5330 bp |
| 完整質粒大小: | -- |
| 載體修飾: | -- |
| 載體類型: | 哺乳動物表達，逆轉錄病毒 |
| 篩選標記: | 新霉素（G418） |
| 高拷貝/低拷貝: | 高拷貝 |
| 生長條件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名稱: | large T antigen,large T cDNA,pBNT,LTc |
| 物種來源: | simian virus |
| 插入大小: | 2200 bp |
| 基因突變: | -- |
| GenBank編號 | -- |
| 啟動子 | -- |
| 標簽/融合蛋白: | -- |
| 克隆方法: | 限制性內切酶 |
| 5' 克隆位點: | BamHI(未破壞) |
| 3' 克隆位點: | BamHI(未破壞) |
| 5' 測序引物 | pBABE 5' |
| 3' 測序引物 | pBABE-3 |
|
文獻引用方法 |
|
| 材料和方法部分: | pBABE-neo largeTcDNA was a gift from Bob Weinberg (Addgene plasmid # 1780 ; //n2t.net/addgene:1780 ; RRID:Addgene_1780) |
| 參考文獻部分: | Enumeration of the simian virus 40 early region elements necessary for human cell transformation. Hahn WC, Dessain SK, Brooks MW, King JE, Elenbaas B, Sabatini DM, DeCaprio JA, Weinberg RA. Mol Cell Biol 2002 Apr;22(7):2111-23. 10.1128/MCB.22.7.2111-2123.2002 PubMed 11884599 |
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pBABE-neo largeTcDNA載體質粒基本信息
pBABE-neo largeTcDNA質粒圖譜載體圖譜和pBABE-neo largeTcDNA載體序列質粒序列多克隆位點信息
pBABE-neo largeTcDNA質粒載體簡介
pBABE-neo largeTcDNA質粒序列載體序列
LOCUS pBABE-neo largeTcDNA 7464 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Retroviral expression of Large T Antigen. Used to create
immortalized cell lines..
ACCESSION .
VERSION .
KEYWORDS pBABE-neo largeTcDNA
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7464)
AUTHORS Hahn WC, Dessain SK, Brooks MW, King JE, Elenbaas B, Sabatini DM,
DeCaprio JA, Weinberg RA
TITLE Enumeration of the simian virus 40 early region elements necessary
for human cell transformation.
JOURNAL Mol Cell Biol 2002 Apr;22(7):2111-23.
PUBMED 11884599
REFERENCE 2 (bases 1 to 7464)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
//www.snapgene.com
FEATURES Location/Qualifiers
source 1..7464
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 6..475
/note="long terminal repeat from Moloney murine leukemia
virus"
misc_feature 540..739
/label=MMLV Psi
/note="packaging signal of Moloney murine leukemia virus
(MMLV)"
CDS 940..1356
/label=gag (truncated)
/note="truncated Moloney murine leukemia virus (MMLV) gag
gene lacking the start codon"
primer_bind 1286..1308
/label=pLXSN 5'
/note="Murine stem cell virus, forward primer. Also called
MSCV"
primer_bind 1324..1340
/label=pBABE 5'
/note="Psi packaging signal, forward primer for pBABE
vectors"
CDS 1394..3520
/codon_start=1
/product="large T antigen from SV40 (simian virus 40)"
/label=large T antigen
/translation="MDKVLNREESLQLMDLLGLERSAWGNIPLMRKAYLKKCKEFHPDK
GGDEEKMKKMNTLYKKMEDGVKYAHQPDFGGFWDATEIPTYGTDEWEQWWNAFNEENLF
CSEEMPSSDDEATADSQHSTPPKKKRKVEDPKDFPSELLSFLSHAVFSNRTLACFAIYT
TKEKAALLYKKIMEKYSVTFISRHNSYNHNILFFLTPHRHRVSAINNYAQKLCTFSFLI
CKGVNKEYLMYSALTRDPFSVIEESLPGGLKEHDFNPEEAEETKQVSWKLVTEYAMETK
CDDVLLLLGMYLEFQYSFEMCLKCIKKEQPSHYKYHEKHYANAAIFADSKNQKTICQQA
VDTVLAKKRVDSLQLTREQMLTNRFNDLLDRMDIMFGSTGSADIEEWMAGVAWLHCLLP
KMDSVVYDFLKCMVYNIPKKRYWLFKGPIDSGKTTLAAALLELCGGKALNVNLPLDRLN
FELGVAIDQFLVVFEDVKGTGGESRDLPSGQGINNLDNLRDYLDGSVKVNLEKKHLNKR
TQIFPPGIVTMNEYSVPKTLQARFVKQIDFRPKDYLKHCLERSEFLLEKRIIQSGIALL
LMLIWYRPVAEFAQSIQSRIVEWKERLDKEFSLSVYQKMKFNVAMGIGVLDWLRNSDDD
DEDSQENADKNEDGGEKNMEDSGHETGIDSQSQGSFQAPQSSQSVHDHNQPYHICRGFT
CFKKPPTPPPEPET"
primer_bind complement(3601..3621)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 3606..3935
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 3786..3921
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 3848..3867
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 4292..5086
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(4346..4365)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 4956..4975
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind 5609..5628
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
LTR 5890..6359
/note="long terminal repeat from Moloney murine leukemia
virus"
CDS complement(6474..7334)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 7097..7116
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(7335..7439)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gcagcctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcagg
61 gccaagaaca gatggaacag ctgaatatgg gccaaacagg atatctgtgg taagcagttc
121 ctgccccggc tcagggccaa gaacagatgg tccccagatg cggtccagcc ctcagcagtt
181 tctagagaac catcagatgt ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt
241 atttgaacta accaatcagt tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc
301 tcaataaaag agcccacaac ccctcactcg gggcgccagt cctccgattg actgagtcgc
361 ccgggtaccc gtgtatccaa taaaccctct tgcagttgca tccgacttgt ggtctcgctg
421 ttccttggga gggtctcctc tgagtgattg actacccgtc agcgggggtc tttcatttgg
481 gggctcgtcc gggatcggga gacccctgcc cagggaccac cgacccacca ccgggaggca
541 agctggccag caacttatct gtgtctgtcc gattgtctag tgtctatgac tgattttatg
601 cgcctgcgtc ggtactagtt agctaactag ctctgtatct ggcggacccg tggtggaact
661 gacgagttct gaacacccgg ccgcaaccct gggagacgtc ccagggactt tgggggccgt
721 ttttgtggcc cgacctgagg aagggagtcg atgtggaatc cgaccccgtc aggatatgtg
781 gttctggtag gagacgagaa cctaaaacag ttcccgcctc cgtctgaatt tttgctttcg
841 gtttggaacc gaagccgcgc gtcttgtctg ctgcagcgct gcagcatcgt tctgtgttgt
901 ctctgtctga ctgtgtttct gtatttgtct gaaaattagg gccagactgt taccactccc
961 ttaagtttga ccttaggtca ctggaaagat gtcgagcgga tcgctcacaa ccagtcggta
1021 gatgtcaaga agagacgttg ggttaccttc tgctctgcag aatggccaac ctttaacgtc
1081 ggatggccgc gagacggcac ctttaaccga gacctcatca cccaggttaa gatcaaggtc
1141 ttttcacctg gcccgcatgg acacccagac caggtcccct acatcgtgac ctgggaagcc
1201 ttggcttttg acccccctcc ctgggtcaag ccctttgtac accctaagcc tccgcctcct
1261 cttcctccat ccgccccgtc tctccccctt gaacctcctc gttcgacccc gcctcgatcc
1321 tccctttatc cagccctcac tccttctcta ggcgccggcc ggatcccgcg accggtcgac
1381 cagctttgca aagatggata aagttttaaa cagagaggaa tctttgcagc taatggacct
1441 tctaggtctt gaaaggagtg cctgggggaa tattcctctg atgagaaagg catatttaaa
1501 aaaatgcaag gagtttcatc ctgataaagg aggagatgaa gaaaaaatga agaaaatgaa
1561 tactctgtac aagaaaatgg aagatggagt aaaatatgct catcaacctg actttggagg
1621 cttctgggat gcaactgaga ttccaaccta tggaactgat gaatgggagc agtggtggaa
1681 tgcctttaat gaggaaaacc tgttttgctc agaagaaatg ccatctagtg atgatgaggc
1741 tactgctgac tctcaacatt ctactcctcc aaaaaagaag agaaaggtag aagaccccaa
1801 ggactttcct tcagaattgc taagtttttt gagtcatgct gtgtttagta atagaactct
1861 tgcttgcttt gctatttaca ccacaaagga aaaagctgca ctgctataca agaaaattat
1921 ggaaaaatat tctgtaacct ttataagtag gcataacagt tataatcata acatactgtt
1981 ttttcttact ccacacaggc atagagtgtc tgctattaat aactatgctc aaaaattgtg
2041 tacctttagc tttttaattt gtaaaggggt taataaggaa tatttgatgt atagtgcctt
2101 gactagagat ccattttctg ttattgagga aagtttgcca ggtgggttaa aggagcatga
2161 ttttaatcca gaagaagcag aggaaactaa acaagtgtcc tggaagcttg taacagagta
2221 tgcaatggaa acaaaatgtg atgatgtgtt gttattgctt gggatgtact tggaatttca
2281 gtacagtttt gaaatgtgtt taaaatgtat taaaaaagaa cagcccagcc actataagta
2341 ccatgaaaag cattatgcaa atgctgctat atttgctgac agcaaaaacc aaaaaaccat
2401 atgccaacag gctgttgata ctgttttagc taaaaagcgg gttgatagcc tacaattaac
2461 tagagaacaa atgttaacaa acagatttaa tgatcttttg gataggatgg atataatgtt
2521 tggttctaca ggctctgctg acatagaaga atggatggct ggagttgctt ggctacactg
2581 tttgttgccc aaaatggatt cagtggtgta tgacttttta aaatgcatgg tgtacaacat
2641 tcctaaaaaa agatactggc tgtttaaagg accaattgat agtggtaaaa ctacattagc
2701 agctgctttg cttgaattat gtggggggaa agctttaaat gttaatttgc ccttggacag
2761 gctgaacttt gagctaggag tagctattga ccagttttta gtagtttttg aggatgtaaa
2821 gggcactgga ggggagtcca gagatttgcc ttcaggtcag ggaattaata acctggacaa
2881 tttaagggat tatttggatg gcagtgttaa ggtaaactta gaaaagaaac acctaaataa
2941 aagaactcaa atatttcccc ctggaatagt caccatgaat gagtacagtg tgcctaaaac
3001 actgcaggcc agatttgtaa aacaaataga ttttaggccc aaagattatt taaagcattg
3061 cctggaacgc agtgagtttt tgttagaaaa gagaataatt caaagtggca ttgctttgct
3121 tcttatgtta atttggtaca gacctgtggc tgagtttgct caaagtattc agagcagaat
3181 tgtggagtgg aaagagagat tggacaaaga gtttagtttg tcagtgtatc aaaaaatgaa
3241 gtttaatgtg gctatgggaa ttggagtttt agattggcta agaaacagtg atgatgatga
3301 tgaagacagc caggaaaatg ctgataaaaa tgaagatggt ggggagaaga acatggaaga
3361 ctcagggcat gaaacaggca ttgattcaca gtcccaaggc tcatttcagg cccctcagtc
3421 ctcacagtct gttcatgatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt
3481 taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg
3541 ttaacgggga tcccagtgtg gtggtacgta ggaattcgcc agcacagtgg tcgaccctgt
3601 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
3661 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
3721 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
3781 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
3841 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
3901 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct tcacgctgcc gcaagcactc
3961 agggcgcaag ggctgctaaa ggaagcggaa cacgtagaaa gccagtccgc agaaacggtg
4021 ctgaccccgg atgaatgtca gctactgggc tatctggaca agggaaaacg caagcgcaaa
4081 gagaaagcag gtagcttgca gtgggcttac atggcgatag ctagactggg cggttttatg
4141 gacagcaagc gaaccggaat tgccagctgg ggcgccctct ggtaaggttg ggaagccctg
4201 caaagtaaac tggatggctt tcttgccgcc aaggatctga tggcgcaggg gatcaagatc
4261 tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg
4321 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg
4381 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa
4441 gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct
4501 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga
4561 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc
4621 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac
4681 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc
4741 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact
4801 gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga
4861 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg
4921 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga
4981 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga
5041 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg
5101 ttcgcatcga tgcatcgatg cggcgggtgt ggtggttacg cgtaatctgc tgcttgcaaa
5161 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
5221 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
5281 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
5341 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
5401 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
5461 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
5521 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
5581 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
5641 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
5701 tatggaaaaa cgccagcaac gcatcgataa aataaaagat tttatttagt ctccagaaaa
5761 aggggggaat gaaagacccc acctgtaggt ttggcaagct agcttaagta acgccatttt
5821 gcaaggcatg gaaaaataca taactgagaa tagagaagtt cagatcaagg tcaggaacag
5881 atggaacagc tgaatatggg ccaaacagga tatctgtggt aagcagttcc tgccccggct
5941 cagggccaag aacagatgga acagctgaat atgggccaaa caggatatct gtggtaagca
6001 gttcctgccc cggctcaggg ccaagaacag atggtcccca gatgcggtcc agccctcagc
6061 agtttctaga gaaccatcag atgtttccag ggtgccccaa ggacctgaaa tgaccctgtg
6121 ccttatttga actaaccaat cagttcgctt ctcgcttctg ttcgcgcgct tctgctcccc
6181 gagctcaata aaagagccca caacccctca ctcggggcgc cagtcctccg attgactgag
6241 tcgcccgggt acccgtgtat ccaataaacc ctcttgcagt tgcatccgac ttgtggtctc
6301 gctgttcctt gggagggtct cctctgagtg attgactacc cgtcagcggg ggtctttcac
6361 atgcagcatg tatcaaaatt aatttggttt tttttcttaa gtatttacat taaatggcca
6421 tagtgcggcc gcaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
6481 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
6541 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
6601 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
6661 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
6721 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
6781 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
6841 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
6901 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
6961 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
7021 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
7081 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
7141 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
7201 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
7261 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
7321 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
7381 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
7441 acatttcccc gaaaagtgcc acct
//
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